Table 1.
Analysis of the changes in spatial distribution of AGP epitopes, detected in fruit after fungal infection (inoculation day and 16th day of fungal inoculation).
| Experiment I | Tissue level | Cellular level | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Epidermal layer | Hypodermal layer | Parenchyma | Cell wall - plasma membrane | Cytoplasm compartments | |||||||
| mAb | Treatment | Treatment | |||||||||
| C | FI | C | FI | C | FI | C | FI | C | FI | ||
| Inoculation day in | JIM13 | + | + | + | + | + | + | + | + | − | − |
| LM2 | + | −/+ | + | −/+ | −/+ | −/+ | + | + | − | − | |
| LM14 | + | + | + | + | + | + | + | + | − | − | |
| MAC207 | − | + | − | + | −/+ | + | −/+ | + | − | − | |
| 16 days 16 | JIM13 | + | ++ | + | ++ | + | ++ | + | ++ | − | ++ |
| LM2 | −/+ | + | −/+ | −/+ | −/+ | −/+ | −/+ | + | − | + | |
| LM14 | + | ++ | + | ++ | + | ++ | + | ++ | − | + | |
| MAC207 | + | + | −/+ | + | − | + | −/+ | + | − | + | |
Immunolabelling was evaluated according to Xie and co-workers (5): (−) no labelling, epitope not detected; (+) middle labelling; (++) very strong fluorescence signal. Abbreviations: C – control sample; FI – fruit after fungal infection
The addition of the β-GlcY caused progression of the infection and thus destruction of the apple tissue, which resulted in an unclear pattern of AGP labelling with all the antibodies used. Analysis at the tissue level showed disorderly changes in AGP localization after 16 days of the experiment over the entire fruit surface. The progress of the fungal disease was correlated with displacement of AGP and disturbance in their uninterrupted occurrence in the cell wall-plasma membrane continuum.