Table 2.
Experiment II | Tissue level | Cellular level | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
Epidermal layer | Hypodermal layer | Parenchyma | Cell wall - plasma membrane | Cytoplasmic remnants | |||||||
mAb | Treatment | Treatment | |||||||||
C | FI | C | FI | C | FI | C | FI | C | FI | ||
Inoculation day in | JIM13 | + | + | + | + | + | + | + | + | − | − |
LM2 | + | + | + | + | −/+ | −/+ | + | + | − | − | |
LM14 | ++ | ++ | ++ | ++ | + | + | + | + | − | − | |
MAC207 | + | + | + | + | −/+ | − | + | + | − | − | |
16 days 16 | JIM13 | + | + | + | ++ | + | ++ | + | ++ | − | ++ |
LM2 | −/+ | −/+ | −/+ | −/+ | −/+ | −/+ | −/+ | −/+ | − | + | |
LM14 | + | −/+ | + | −/+ | + | −/+ | + | −/++ | − | + | |
MAC207 | + | −/+ | + | −/+ | + | −/+ | + | −/+ | − | − |
Immunolabelling was evaluated according to Xie and co-workers (5): (−) no labelling, epitope not detected; (+) middle labelling; (++) very strong fluorescence signal. Abbreviations: C – control sample; FI – fruit after fungal infection.