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. 2019 Nov 22;9:17402. doi: 10.1038/s41598-019-53192-4

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Shh-nlacZ expression in the hippocampus and cerebellum was restricted to GABAergic interneurons. (A) Panoramic view of cerebellum folia showing the expression of Shh-nlacZ in GABAergic Purkinje neurons (25X, bar 100 µm). (B–F) Overall view of CA1 and CA3 regions of the hippocampus showing expression of Shh-lacZ restricted to sparse GABAergic cells. The cellular sub-type was not CR, PV, NPY or STT positive (remains unknown). All imagines were 25X (B and D bar = 100 µm) (C, E and F bar = 50 µm). Abbr: SR, stratum radiatum; SP, stratum pyramidale; SO, stratum oriens; SL Stratum lacunosum; CA1, Cornu Ammonis-1; CA3, Cornu Ammonis- 3.