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. 2019 Nov 22;9:17394. doi: 10.1038/s41598-019-54004-5

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Top: The average number of genes in the ST131 pangenome (y-axis) increased as the 4,071 genomes were added (x-axis) indicating an open pangenome for the whole collection (black), as well as its clades and subclades: Clade A (blue), Clade B (green), Clade C (grey), subclade C1_6 (red), subclade C2_4 (orange) and subclade C2_9 (brown). Below: Alpha varied with numbers of genomes sampled (shown here for >30 genomes) and was more independent from sample number once the number of genomes examined about >250. Note that the x-axis’ log₁₀ scale.