Corneal fluorescein staining of naïve and injured eyes was performed, and epithelial defects were evaluated by slit lamp biomicroscopy with cobalt blue light. (A) Representative images of fluorescein-stained corneas at days 0, 2, and 4 post-injury. The green areas represent epithelial defects. (B) Bar graph showing area of epithelial defect at days 2 and 4 post-injury (relative to day 0, set as 100%). Data from three independent experiments are shown, and each experiment consisted of 3–5 animals. The values are shown as mean ± SD. *p<0.05 as compared to untreated injured control.