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. Author manuscript; available in PMC: 2020 Sep 20.
Published in final edited form as: J Mol Biol. 2019 Jul 13;431(20):4007–4029. doi: 10.1016/j.jmb.2019.07.017

Figure 8. NusG- and RfaH-bound EC structures.

Figure 8.

RNAP subunits and nucleic acids are labelled. A. E. coli NusG (colored in green) binds between β and β’ subunits, contacting β protrusion, β lobe, and β’ clamp helices. NusG KOW domain was disordered in the cryo-EM structure. B. E. coli RfaH (colored in orange) binds to the same site as NusG in panel A. In addition, RfaH recognizes a short-hairpin formed by ops sequence in the non-template DNA (not visible in the figure) and RfaH KOW domain binds flap tip of RNAP, covering upstream duplex DNA near the transcriptpion bubble.