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. 2019 Oct 14;22(2):203–211. doi: 10.22074/cellj.2020.6699

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The Cell Journal (Yakhteh) is an open access journal which means the articles are freely available online for any individual author to download and use the providing address. The journal is licensed under a Creative Commons Attribution-Non Commercial 3.0 Unported License which allows the author(s) to hold the copyright without restrictions that is permitting unrestricted use, distribution, and reproduction in any medium provided the original work is properly cited.

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Fig 5 — Real-time polymerase chain reaction (PCR) and western blotting analysis of CDKN2A/B in the pancreatic islets of the control and offspring of gestational diabetes (OGD) rats. CDKN2A/B mRNAs were determined by quantitive PCR (qPCR). A. Reverse transcription polymerase chain reaction (RT-PCR) analysis for the mRNA expression of CDKN2A/B, B. Real-time PCR analysis for the mRNA level of CDKN2A/B in control and OGD samples, C, D. Densitometric quantiﬁcation of western blotting bands for P15 and P16 proteins in the two groups, and E. Nitrocellulose blot of sodium dodecyl sulfate polyacrylamide gel electrophoresis SDS-PAGE gel developed with immune-blot assay kit, column 1, control group; column 2, OGD group. GAPDH was used as the housekeeping gene. Data are presented as means ± SD, and the experiments were repeated independently three times. *; P<0.05 and **; P<0.01.