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. Author manuscript; available in PMC: 2020 Dec 1.
Published in final edited form as: Hum Mutat. 2019 Sep 15;40(12):2414–2429. doi: 10.1002/humu.23898

Table 1.

PARN kinetic rate constants

kobs1 kobs2

Divalent metal ion PARN poly-peptide Rate kobs1 x 103 (s−1) Catalytic efficiency x 105 (s−1 M−1) Catalytic efficiency ratio WT/Y91C Rate kobs2 x 103 (s−1) Catalytic efficiency x 105 (s−1 M−1) Catalytic efficiency ratio WT/Y91C Rate ratio kobs1/ kobs2

Mg2+ WT 22 ± 6 7 ± 2 30 0.2 ± 0.002 0.07 ± 0.001 31 109
Y91C 2 ± 0.9 0.2 ± 0.09 0.02 ± 0.005 0.002 ± 0.0005 111

Mn2+ WT 14 ± 0.5 5 ± 0.2 27 27 ± 0.1 9 ± 0.04 30 0.5
Y91C 2 ± 0.9 0.2 ± 0.1 3 ± 1 0.3 ± 0.1 0.6