Table 1.
kobs1 | kobs2 | |||||||
---|---|---|---|---|---|---|---|---|
Divalent metal ion | PARN poly-peptide | Rate kobs1 x 103 (s−1) | Catalytic efficiency x 105 (s−1 M−1) | Catalytic efficiency ratio WT/Y91C | Rate kobs2 x 103 (s−1) | Catalytic efficiency x 105 (s−1 M−1) | Catalytic efficiency ratio WT/Y91C | Rate ratio kobs1/ kobs2 |
Mg2+ | WT | 22 ± 6 | 7 ± 2 | 30 | 0.2 ± 0.002 | 0.07 ± 0.001 | 31 | 109 |
Y91C | 2 ± 0.9 | 0.2 ± 0.09 | 0.02 ± 0.005 | 0.002 ± 0.0005 | 111 | |||
Mn2+ | WT | 14 ± 0.5 | 5 ± 0.2 | 27 | 27 ± 0.1 | 9 ± 0.04 | 30 | 0.5 |
Y91C | 2 ± 0.9 | 0.2 ± 0.1 | 3 ± 1 | 0.3 ± 0.1 | 0.6 |