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. Author manuscript; available in PMC: 2020 Dec 1.
Published in final edited form as: Hum Mutat. 2019 Sep 10;40(12):2393–2413. doi: 10.1002/humu.23895

Figure. 7. M2 pore loop variants influence calcium permeability.

Figure. 7

(A,B) Current-voltage (I-V) relationships in an external solution containing high Na+ (140 mM) plus 0 mM Ca2+ (open circles) or 10 mM Ca2+ (solid circles) for WT GluN1/GluN2A (A, left panel) or WT GluN1/GluN2B (B, left panel) or M2 loop variants in the same background. The Ca2+-free I-V is the average of that recorded before and after the 10 mM Ca2+ recording. We used the measured reversal potentials to calculate PCa/PNa (see Methods).

(C,D) Mean (± SEM) relative calcium permeability (PCa/PNa) was calculated from reversal potentials for (C) GluN1/GluN2A (n = 11), GluN1-p.G620R/GluN2A (n = 4), GluN1/GluN2A-p.L611Q (n = 6), and GluN1/GluN2A-p.N615K (n = 5) and (D) WT GluN1/GluN2B (n = 6), GluN1/GluN2B-p.N615I (n = 6), GluN1/GluN2B-p.V618G (n = 5), and GluN1/GluN2B-p.V620M (n = 6). M2 variant values are significantly different from their respective wild type (* p < 0.05, one way ANOVA, with Dunnett multiple comparisons). Fitted and statistical parameters are given in Table 5 and Supp. Table S4.