Skip to main content

View full-text article in PMC

. Author manuscript; available in PMC: 2020 Dec 1.

Published in final edited form as: Hum Mutat. 2019 Sep 10;40(12):2393–2413. doi: 10.1002/humu.23895

Table 3.

Summary of pharmacological data

	Glutamate EC₅₀, μM (n)	Glycine EC₅₀, μM (n)	%, pH6.8/pH7.6^γ
WT GluN1/WT 2A	4.9 ± 0.35 (26)	1.1 ± 0.06 (23)	44 ± 1.8 (29)
1-G618R/2A	4.8 ± 0.28 (10)	1.7 ± 0.07 (9)^*	38 ± 3.1 (12)
1-G620R/2A	6.2 ± 0.42 (11)	1.3 ± 0.10 (12)	25 ± 1.8 (10)^*
2A-L611Q	2.5 ± 0.16 (21)^*	0.83 ± 0.08 (16)^*	63 ± 2.3 (11)^*
2A-N614S	2.6 ± 0.38 (11)^*	0.61 ± 0.03 (11)^*	57 ± 2.8 (12)^*
2A-N615K	2.8 ± 0.23 (12)^*	0.75 ± 0.08 (13)^*	44 ± 1.8 (10)

WT GluN1/WT 2B	1.5 ± 0.09 (25)	0.43 ± 0.04 (40)	17 ± 0.7 (31)
1-G618R/2B	1.3 ± 0.10 (12)	n.a.	21 ± 1.4 (15)^*
1-G620R/2B	1.9 ± 0.22 (12)	0.43 ± 0.03 (9)	18 ± 1.2 (10)
2B-W607C	2.0 ± 0.22 (12)^*	0.44 ± 0.05 (10)	11 ± 0.70 (12)^*
2B-G611V	1.7 ± 0.33 (12)	0.28 ± 0.04 (8)	12 ± 2.0 (10)
2B-N615I	1.4 ± 0.16 (10)	0.26 ± 0.02 (11)^*	19 ± 0.6 (6)
2B-N615K	1.3 ± 0.16 (11)	0.22 ± 0.03 (12)^*	16 ± 3.8 (7)
2B-N616K	1.1 ± 0.19 (8)	0.14 ± 0.01 (7)^*	17 ± 1.7 (12)
2B-V618G	1.6 ± 0.15 (8)	0.47 ± 0.02 (15)	10 ± 0.68 (10)^*
2B-V620M	1.1 ± 0.11 (12)	0.58 ± 0.07 (21)	24 ± 1.8 (10)^*

Data were generated by oocytes recordings with −40 mV of holding potential, and were expressed as Mean ± SEM (n).

^γ

percentage of the current at pH 6.8 compared to that at pH 7.6.

^*

p < 0.05 LogEC₅₀ compared by one way ANOVA, with Dunnett’s multiple comparisons test.

Statistical analysis is given in Supp. Table S2.