Figure - PMC

Skip to main content

View full-text article in PMC

. Author manuscript; available in PMC: 2020 Dec 1.

Published in final edited form as: Hepatology. 2019 Aug 13;70(6):1986–2002. doi: 10.1002/hep.30780

Fig. 6 — Male and female Smad158;Alb-Cre+ and littermate Cre- control mice were sacrificed at 8 weeks of age (n=6–8 per group). Serum was collected to measure (A) alanine aminotransferase (ALT) and (B) total bilirubin. (C, F) Liver malondialdehyde, hydroxyproline, and Col1a1 relative to Rpl19 mRNA levels were quantified by colorimetric assays or qRT-PCR. (D) Liver sections from a subset of mice (n=4 per group) were stained with hematoxylin and eosin, picrosirius red for collagen formation, or Perls’ Prussian blue for tissue iron. Branches of the central vein (C) and ceroid-laden macrophages (arrows) are indicated. Images were taken under the brightfield and/or polarized light, and representative images are shown. (E) The sirius red positive area was quantitated by dividing total area calculated in polarized light (after subtraction of major veins) by total area calculated under the brightfield (n=4 per group). Data are presented as box plots with min to max whiskers. For qRT-PCR, the average of male Cre- control mice was set to 1. *P < 0.05, **P < 0.01, ***P < 0.001 relative to their respective Cre- controls by Student’s t test.

Fig. 6 — Male and female Smad158;Alb-Cre+ and littermate Cre- control mice were sacrificed at 8 weeks of age (n=6–8 per group). Serum was collected to measure (A) alanine aminotransferase (ALT) and (B) total bilirubin. (C, F) Liver malondialdehyde, hydroxyproline, and Col1a1 relative to Rpl19 mRNA levels were quantified by colorimetric assays or qRT-PCR. (D) Liver sections from a subset of mice (n=4 per group) were stained with hematoxylin and eosin, picrosirius red for collagen formation, or Perls’ Prussian blue for tissue iron. Branches of the central vein (C) and ceroid-laden macrophages (arrows) are indicated. Images were taken under the brightfield and/or polarized light, and representative images are shown. (E) The sirius red positive area was quantitated by dividing total area calculated in polarized light (after subtraction of major veins) by total area calculated under the brightfield (n=4 per group). Data are presented as box plots with min to max whiskers. For qRT-PCR, the average of male Cre- control mice was set to 1. *P < 0.05, **P < 0.01, ***P < 0.001 relative to their respective Cre- controls by Student’s t test.