Table 1: Administration of 20 kDa free PEG does not affect clinical blood chemistry or urinalysis results.
PEG 20 kDa (in mice injected with APA)
Naïve mice were given exogenous APA (30 μg per mouse) or PBS, followed 24hr later by an injection of 20 kDa free PEG (550 mg/kg). Mice were given one more injection of PBS or PEG at day 7 and were sacrificed at day 14. Terminal blood was collected via cardiac puncture. Complete blood counts were performed within 60 minutes of sample collection, and serum/urine tests were performed according to manufacturer recommendations.
| PBS + PBS (n=10) | APA + PEG (n=10) | p-value | ||
|---|---|---|---|---|
| Complete Blood Count | ||||
| Red blood cell(M/μL) | 10.8 | 10.6 | 0.08 | |
| Hemoglobin (g/dL) | 16.6 | 16.3 | 0.12 | |
| Hematocrit (%) | 48.7 | 47.7 | 0.15 | |
| MCV (fL) | 44.5 | 45.1 | 0.56 | |
| Platelet (K/μL) | 114.4 | 113.4 | 0.96 | |
| Reticulocyte (K/μL) | 457.7 | 477.4 | 0.54 | |
| WBC (K/uL) | 6.3 | 6.8 | 0.46 | |
| Neutrophil # (K/μL) | 1.3 | 1.4 | 0.56 | |
| Lymphocyte # (K/μL) | 4.5 | 5.0 | 0.39 | |
| Serum | ||||
| Creatinine (mg/dL) | 0.38 | 0.39 | 0.91 | |
| Albumin (g/dL) | 2.9 | 3.0 | 0.68 | |
| BUN (mg/dL) | 22.1 | 21.8 | 0.83 | |
| Urine | ||||
| Creatinine (mg/dL) | 65.2 | 64.5 | 0.92 | |
| Total protein (mg/dL) | 203.1 | 161.3 | 0.10 |