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. 2019 Nov 6;2019:5069578. doi: 10.1155/2019/5069578

Figure 2.

Figure 2

White adipocyte differentiation impaired by microRNA mimic-mediated overexpression of miR-129-5p. (a) Expression levels of miR-129-5p. (b) Oil Red O staining of mature adipocytes. The top two images were captured by a camera; the lower two images were acquired with a microscope at 100x amplification. (c) Relative TG content of cells isolated from EWAT. (d) The time course of miR-129-5p expression during normal adipogenic differentiation was detected, and related white adipogenic genes were qualified by RT-qPCR. (e) Protein levels of the target genes were determined by western blot. (f) Densitometry quantification of western blot. The results of Student's t-test are presented as mean ± SEM of a representative of more than three independent experiments (∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001).