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. 2019 Nov 6;2019:5069578. doi: 10.1155/2019/5069578

Figure 5.

Figure 5

ATG7 was directly downregulated by miR-129-5p in vitro. (a) Predicted target genes were validated by RT-qPCR in SVF white adipocytes. (b) Predicted target genes were validated by RT-qPCR in beige adipocytes. (c) miR-129-5p regulatory element in the 3′UTR of mouse ATG7 was identified by Targetscan. MRE, miRNA regulatory element; MRE-wt, wild-type MRE; MRE-mut, mutated MRE. (d) The effect of miR-129-5p mimics on the reporter construct containing ATG7-3′UTR in MRE-wt or MRE-mut as determined in HEK 293T cells. Relative luciferase units (RLU) are shown. (e–g) ATG7 and LC3I/II were determined by western blot transfected control and miR-129-5p mimics in mature white, beige, and brown adipocytes from SVF. Error bars represent SEM of more than three independent experiments (∗∗p < 0.01 and ∗∗∗p < 0.001).