Table 1.
Effects of noreugenin (NRG) or α-hydroxy-butein (AH-BU) on myeloperoxidase activity of the neutrophils obtained from fluid leakage from the mouse peritoneal cavity 4 h after an intraperitoneal injection of oyster glycogen solution.
| Groups | Concentrations (μm) | MPO (mU/mL) (% of inhibition) |
|---|---|---|
| Control | 371.10 ± 3.30 | |
| LPS | 437.80 ± 5.80 | |
|
| ||
| Noreugenin | 1 | 423.10 ± 4.21 |
| 2.5 | 417.30 ± 7.53 | |
| 5 | 391.90 ± 10.76(10.47±2.46%)∗∗ | |
| 10 | 373.90 ± 11.48(14.60±2.62%)∗∗ | |
| 50 | 369.20 ± 5.32(15.68±1.21%)∗∗ | |
|
| ||
| α-Hydroxy-butein | 1 | 418.40 ± 6.92 |
| 2.5 | 418.40 ± 6.88 | |
| 5 | 379.90 ± 7.82(13.23±1.79%)∗∗ | |
| 10 | 371.00 ± 7.50(15.26±1.71%)∗∗ | |
| 50 | 365.80 ± 3.15(16.46±0.72%)∗∗ | |
MPO: myeloperoxidase; LPS: lipopolysaccharide. Cells were pretreated with different concentrations of noreugenin (NRG: 1–50 μM) or α-hydroxy-butein (AH-BU: 1–50 μM) for 0.5 h and then stimulated with LPS (5 μg/mL) for 18 h. Control = cells treated with sterile phosphate-buffered saline (PBS, pH 7.6) only. LPS = cells treated with LPS (5 μg/mL) only. Each group represents the mean ± S.E.M. of experiments conducted in triplicate from three to six animals. ∗∗p < 0.01 compared to the positive control group (LPS); ANOVA/Newman–Keuls test.