Figure 4.

High 4-HNE-protein conjugates in aqueous and vitreous humors of patients with synchysis scintillans as compared to other vitreoretinal diseases. (a) Representative pattern of 4-HNE-protein conjugates extracted from the vitreous and aqueous humors of patients affected by idiopathic epimacular membrane or synchisis scintillans. Protein extracts were electrophoretically separated and western blotted, using antibody against anti-4-HNE-conjugates: purified human serum albumin (HSA) and 4-HNE-conjugated HSA (4-HNE-mod HSA) were used as negative and positive controls, respectively, to test the specificity of the method. The protein bands of the analyzed humors were quantified (see Section 2), and the values, expressed as arbitrary units, are represented in (b) as means ± SEM. Significant differences are shown by ∗ at p < 0.01.