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. 2019 Nov 6;2019:1845926. doi: 10.1155/2019/1845926

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Copyright © 2019 Ziang Zhang et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Cellular morphology and proliferation of ASCs cultured on different substrates. (a) Cellular morphology of ASCs cultured on different substrates. ASCs were reseeded on three different substrates: growth d-ECM, adipogenic d-ECM, and tissue culture polystyrene (TCP). Immunostaining for F-actin. (b) ASC morphology is quantitatively compared by measuring cell aspect ratio using ImageJ software. (c) EdU assay for proliferation of ASCs. (d) Quantitative analysis of EdU⁺ cell proliferation rate. (e) Colony-forming assay. (f) Quantitative analysis of colony number per well. Results are presented as the mean ± SD; one-way ANOVA followed by Bonferroni's post hoc test analysis for multiple comparison. Scale bar = 100 μm.