Figure 5.

Knocking down Nrf2 expression attenuated the effects of LXA4 on the regulation of proinflammatory factors and ROS in HPMECs. (a) Nrf2 mRNA levels in HPMECs. The cells were transfected with a Nrf2 lentivirus for 72 h. The mRNA levels of Nrf2 were determined by RT-PCR. (b–d) Determination of IL-1β, IL-6, and E-selectin mRNA expression by RT-PCR. β-Actin served as an internal control. (e) Cellular ROS levels in the Nrf2 knockout HPMECs, measured using DHE. This experiment was independently repeated three times with 3 wells per time. (f) The quantitative mean fluorescence intensity in each treatment group is shown (^∗P < 0.05, ^∗∗∗P < 0.001). TNF-α: the TNF-α group; TNF-α+LXA4: the TNF-α+Lipoxin A4 group; LV Nrf2: the Nrf2 lentivirus-transfected group.