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. 2019 Nov 6;2019:2197017. doi: 10.1155/2019/2197017

Figure 6.

Figure 6

Knocking out Nrf2 partially abolished the anti-inflammatory and antioxidative effects of LXA4 on mice. Nrf2−/− mice in the AP group were intraperitoneally injected with caerulein (50 μg/kg, 7 times, ip) and LPS (10 mg/kg, ip), and those in the AP+LXA4 group were injected with LXA4 (0.1 mg/kg) before caerulein+LPS administration following the same protocol used for the Nrf2+/+ mice. (a, d) Representative morphological analysis of the pancreas and lung tissue of the Nrf2−/− mice in the control, AP, and AP+LXA4 groups, (b, e) histological scores of the pancreas and lungs, (c) serum amylase levels, and (f) lung wet/dry weight ratios. (g–i) The levels of TNF-α, IL-1β, and E-selectin in the serum of mice measured by ELISA. (j) Images of IL-6 expression in lung tissue sections assessed by immunohistochemistry. (k) Representative images of the flow cytometric evaluation of cellular ROS levels in lung tissue from each treatment group. (l) Fluorescence signals detected using a flow cytometer. The mean fluorescence intensity values are graphed (∗P < 0.05). AP: the acute pancreatitis group; AP+LXA4: the acute pancreatitis+Lipoxin A4 group.