Table 1.
PCR primer sequences and PCR conditions.
| Primer | Sequence (5′>3′) | Annealing temperature | Number of cycles |
|---|---|---|---|
| ERp57 forward ERp57 reverse |
AGAGACTTGCACCTGAGTAT GCAGTCCTAGGTCCATCATA |
52°C | 28 |
| XBP1s forward XBP1s reverse |
GAGTCCGCAGCAGGTG CCGCCAGAATCCATGGGG |
52°C | 30 |
| GAPDH forward GAPDH reverse |
GAAGGTGAAGGTCGGAGTCAAC CAGAGTTAAAAGCAGCCCTGGT |
55°C | 25 |
| BiP forward BiP reverse |
GGGGACCACCTACTCCTGCGTC ATAGGACGGCGTGATGCGGT |
58°C | 25 |
| Chop forward Chop reverse |
TGTTAAAGATGAGCGGGTGGCA TGCTTTGGTGCTGCTTTCAGGTGT |
58°C | 25 |
| ATF6 forward ATF6 reverse |
TTATCAGTTTACAACCTGCA CTAGGGACTTTAAGCCTCTG |
50°C | 30 |