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. 2019 Oct 29;2019:6276254. doi: 10.1155/2019/6276254

Figure 3.

Figure 3

IL-6 negatively regulates IL-22Rα expression on epidermal keratinocytes. Primary keratinocytes from IL-6KO mice were treated with rmIL-6 for 4/24 hours (mRNA/protein expression) at the indicated concentrations. The expression of IL-22Rα mRNA was analyzed and normalized to 28S ribosomal RNA as control (a). IL-6KO keratinocytes were grown to confluency on multichamber slides. Immunohistochemical analysis of keratinocyte culture stained for the expression of IL-22Rα (green), and nuclear staining DAPI (blue). Representative fluorescent images are shown at 20x (b–e) and 40x (f–i). Quantification of IL-22Rα expression as determined by ImageJ (NIH) is presented (j and k). Data are mean ± SD. Significantly different from 0 ng/ml rmIL-6 (p ≤ 0.05, n = 3 separate experiments).