Figure 2.
Effect of different concentrations of CdrV and CBr in LD formation in isolated macrophages. (a) Effect of CdrV and (b) CBr on LD formation in macrophages incubated with selected concentrations of CBr or RPMI (control) for 1 h. (c) Timecourse of CdrV and (d) CBr induced LD formation. Elicited macrophages were incubated with CdrV (10 μg/mL), CBr (6.5 μg/mL), or RPMI (control) for 1, 3, 6, or 12 h. LDs were quantified using light microscopy after OsO4 staining. Each bar represents the mean ± SEM of the number of LDs/cell in 50 cells. Values represent the mean ± SEM of three independent experiments with 3 to 5 animals ∗p < 0.05 compared with control cells (ANOVA).