Table 3:
Reference values of the ratios of EGFR copies to reference gene copies.
| Component | Cell line | Vial color | EGFR ratio | 95% PCI | 95% PPI | Reference genes used for analysis |
|---|---|---|---|---|---|---|
| A | A-431 | White | 6.4 | 6.2–6.7 | 6.0–6.8 | EIF5B, RPS27A, DCK, PMM1 |
| B | BT-20 | Clear | 5.5 | 5.2–5.7 | 5.1–5.9 | EIF5B, RPS27A, DCK |
| C | C32 | Yellow | 0.78 | 0.75–0.82 | 0.73–0.84 | EIF5B, RPS27A, DCK, PMM1 |
| D | Daoy | Blue | 2.2 | 2.1–2.3 | 2.0–2.3 | EIF5B, RPS27A, PMM1 |
| E | Hs 746T | Red | 1.34 | 1.29–1.41 | 1.25–1.45 | RPS27A, DCK, PMM1 |
| F | SNU-5 | Green | 1.8 | 1.8–1.9 | 1.7–2.0 | EIF5B, RPS27A, DCK |
| GM24385a | NA | 0.94 | 0.90–0.98 | 0.89–1.00 | EIF5B, RPS27A, DCK, PMM1 |
a
GM24385 is not a component of RM8366, it was used to validate the assays. PCI, posterior credible, interval; PPI, prediction interval.