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. Author manuscript; available in PMC: 2020 Dec 1.
Published in final edited form as: Kidney Int. 2019 Aug 30;96(6):1346–1358. doi: 10.1016/j.kint.2019.07.026

Figure 8: Ferric citrate administration in mice with early CKD improves cardiac outcomes and survival.

Figure 8:

Echocardiography analysis of left ventricular ejection fraction (A), stroke volume (B) and cardiac output (C) and M-mode echocardiography (D). Data are presented as mean ± SE, n≥8 per group, p<0.05 vs.* WT-Ctr, $ WT-FC, & Col4a3KO-Ctr mice. mRNA expression of cardiac markers of hypertrophy ANP (E), BNP (F) and Myh7 (G)and FGF23 signaling Fgfr1 (H), Fgfr2 (I), Fgfr3 (J), Fgfr4 (K), Egr1 (L). Data are presented as mean ± SE, n≥3 per group, p<0.05 vs.* WT-Ctr, $ WT-FC, & Col4a3KO-Ctr mice. (M) Representative immunoblots of heart protein extracts stained for MEK/ERK and PLCγ/calcineurin/NFAT pathways activity. Measurements performed in 10 week old mice fed a mineral sufficient diet (Ctr) supplemented or not with 5% ferric citrate (FC) during 6 weeks. (N) Representative immunoblots of heart protein extracts for MEK/ERK and PLCγ/calcineurin/NFAT pathways from 10 week old WT mice collected two hours post-injection of either 0.9% sodium chloride (Ctr), 25 μg/g ferric citrate (FC), 50 μg/g of sodium monobasic phosphate (Pi) or 50 ng/g of FGF23. mRNA expression of cardiac markers FGF23 signaling Fgfr1 (O), Fgfr2 (P), Fgfr3 (Q), Fgfr4 (R), Egr1 (S), Nfat1 (T), Nfat2 (U), Nfat3 (V), Nfat4 (W). Data are presented as mean ± SE, n≥3 per group, p<0.05 vs.* WT-Ctr, $ WT-FC, & WT-Pi mice. (X) Cumulative proportion surviving of Col4a3KO mice fed a mineral sufficient diet (Ctr) supplemented or not with 5% ferric citrate (FC) starting at 4 weeks of age.