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. Author manuscript; available in PMC: 2019 Nov 25.
Published in final edited form as: Sci Transl Med. 2018 Oct 10;10(462):eaat9892. doi: 10.1126/scitranslmed.aat9892

Fig. 1. A viral strategy efficiently generates viable Piezo2 mosaic knockouts.

Fig. 1.

(A) Cartoon depicting our strategy for simultaneously knocking out Piezo2 and marking neurons: A viral vector encoding Cre recombinase was introduced to new born (P0-P2) Piezo2cKO (Piezo2fl/fl/GCaMP+/+) mice; controls were GCaMP6f+/+. Mice were tested at > 8 weeks. (B and C) Representative confocal images of dorsal root ganglia from Piezo2cKO. (B) Green fluorescent protein antibody staining to reveal the GCaMP6f-positive cells (green) and the isolectin IB4 (magenta) to mark putative mechanonociceptors. (C) Representative confocal images of multilabel in situ hybridization; arrowheads highlight multilabeled cells. (D) Quantification of marker positive cells expressing GCaMP6f (N = 5 mice). Scale bars, 50 μm (B) and 25 μm (C).