Table 2.
Thermal stability of PDI and PDI deletion constructs under reducing or oxidizing conditions. Values of the transition temperature and enthalpy representing the ranges of two independent experiments per condition were derived from one-step unfolding profiles as shown in the experiments of Figures 1 and 7.
| Tm | ΔH | |||
|---|---|---|---|---|
| Construct | Reduced | Oxidized | Reduced | Oxidized |
| PDI | 54°C | 48-50°C | 31-35 | 30-32 |
| bb'xa'c | 50-51°C | 42°C | 30-46 | 20-26 |
| abb'x | 62-63°C | 51-52°C | 60-66 | 40 |
| bb'x | 58-60°C | 56°C | 50-66 | 50-66 |