Table 3.

Conformational resilience of PDI and PDI deletion constructs under reducing (r) or oxidizing (o) conditions. Because redox and the individual domains of PDI influence its thermal stability, we could not heat all constructs to a single temperature in order to reach a common state of denaturation. The listed temperatures represent the minimal thermal treatment required for near-complete denaturation. The extent of denaturation and renaturation for each construct (± range) was calculated from two independent experiments represented in Figures 2 and 8.

Construct	Denaturation Temperature	% Folded Structure after Denaturation	% Folded Structure after Renaturation
rPDI	65°C	2 ± 1	85 ± 2
oPDI	70°C	12 ± 4	62 ± 5
rbb'xa'c	55°C	12 ± 8	90 ± 0
obb'xa’c	55°C	11 ± 8	90 ± 0
rabb'x	65°C	6 ± 6	96 ± 4
oabb'x	60°C	14 ± 5	72 ± 8
rbb'x	95°C	8 ± 8	74 ± 5
obb'x	70°C	0 ± 0	88 ± 3