Fig. 4.

Data summary of transgenic ZMM28 protein–protein interactions, transcription modulation, and analyzed direct targets. (A) Summary of protein–protein interactions with potential contribution to transgenically expressed zmm28 mechanism of action. For context, DP202216 V6 maize leaf RNA-seq protein interactor expression values are normalized to zmm28 expression. (B) GO term enrichment of DP202216 V6 leaf tissue transcriptomic analysis. (C) Pathway analysis of differentially expressed gene transcripts in event DP202216 V6 leaf tissue. The heat map displays Log₂ fold change of differentially expressed genes functioning in GO term enriched pathways. GO terms 0015979, 0009765, 0019684, 0006091, 0033013, 0034357, 0044436, and 0009579 are grouped as “photosynthesis related.” (D) Summary of promoter direct target analysis and expression in V6 leaves of event DP202216. BiFC, maize protoplast BiFC; HY1H, heterodimer yeast 1-hybrid. Superscript numbers are as follows: 1, P is biological process, F is molecular function, and C is cellular component; 2, number of genes associated with each GO term that are differentially expressed between WT and event DP202216 V6 leaf; 3, total number of genes in each GO category expressed in V6 leaf total detected transcripts; 4, Fisher’s exact test for GO term enrichment; 5, false discovery rates; and 6, V6 leaf. Asterisk indicates statistically significant (adjusted P < 0.05).