Fig. 2.
Drought severity correlates with perturbations of global rhythm, while heat shock has dramatic impact on soil-grown soybean seedlings. (A) Normalized expression levels of time-indicating genes in control and mild drought-treated samples. Treatments were applied at ZT0, and samples were harvested at the indicated times. Mean and SE are plotted. SEs of some data points are smaller than the size of the data symbol, so they may appear invisible. (B) Estimated Phase24 has a good linear relationship with reported sampling time. Linear regression was performed using estimated Phase24 and reported sampling time. Adjusted R2 = 0.9999 for both control and drought-treated samples. y = x is plotted as a reference. (C) Severe drought stress perturbs global rhythm and causes a dramatic phase shift. (D) Heat shock stress (30-min heat treatment at 42 °C) perturbs global rhythm dramatically. Mean and SE are plotted. ****P < 0.0001 (Student’s t test). A and B are derived from GSE69469. C is derived from GSE40627. D is derived from GSE26198.