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. 2019 Nov 22;18:1533033819879905. doi: 10.1177/1533033819879905

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© The Author(s) 2019

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (http://www.creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 4. — Vascular endothelial growth factor upregulates EZH2 expression of HUVECs. A, Relative qRT-PCR analysis of EZH2 and VASH1. GAPDH serve as reference control. B, Enhancer of zeste homolog 2 and VASH1 were detected using Western blotting after treatment of HUVECs with CM-CAFs, VEGF, or CM-CAFs + sh-VEGF. β-actin was used as reference control. All data were represented as the mean ± SD from at least triplicate experiments.*P < .05,**P < .01, and ***P < .001. CAFs indicates cancer-associated fibroblasts; CM, conditioned medium; EZH2, enhancer of zeste homolog 2; HUVEC, human umbilical vein endothelial cell; qRT-PCR, Quantitative real-time polymerase chain reaction; SD, standard deviation; VASH1, vasohibin 1; VEGF, vascular endothelial growth factor.