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Rhes regulates basal mitophagy. (A) Western blots from GFP-transfected or GFP-Rhes−transfected striatal neuronal cells treated with vehicle or cycloheximide (CHX, 100 µM) at indicated time point. Blots were probed with indicated antibodies. (B) Bar graph shows quantification of band intensities of indicated proteins in A. Protein levels were normalized to actin (n = 3, Student’s t test; *P < 0.05, **P < 0.01, ***P < 0.001; data are mean ± SEM).
