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. 2019 Nov 7;116(47):23724–23734. doi: 10.1073/pnas.1906643116

Fig. 1.

Fig. 1.

LAMP2 expression in the retina and the RPE in young and aged mice. (A) Western blot analyses of LAMP2 expression in the retina or the RPE/choroid from WT or Lamp2 KO mice. LAMP2 was abundantly expressed in the RPE/choroid of WT mice, but was completely absent in the RPE/choroid of Lamp2 KO mice. In contrast, relatively less LAMP2 was observed in the retina of WT mice (band indicated by arrowhead above 75 kDa). ***P < 0.001. One-way ANOVA with post hoc Tukey honestly significant difference (HSD) test. (B) Western blot analysis of LAMP2 expression in the isolated RPE from young or old (2- or 12-mo-old) WT mice. LAMP2 expression significantly decreased with age as normalized by GAPDH or the RPE-specific marker RPE65. (C) SQSTM1 expression notably increased in the RPE/choroid of aged mice compared to those of young mice. Lysates of mice retinas and the RPE/choroid were collected from 6 mice per group for Western blot. Ratiometric analyses were performed with at least 3 times repetitive immunoblotting. **P < 0.001, ***P < 0.0001. Student t test. Values are expressed as mean ± SD.