Fig. 1.

Hyperglycemia induces SOD2 suppression and oxidative stress. Human ACS-5003 neurons were infected with empty (EMP), SOD2 overexpression (↑SOD2), or SOD2 knockdown (shSOD2) lentivirus for 1 d and then treated with either 5 mM low glucose (LG) or 25 mM high glucose (HG) for 4 d in the presence of 1% serum; the cells were then harvested for further analysis. (A) The mRNA levels for gene expression of SOD2 and ERβ (n = 4). (B) Quantitation of protein levels (n = 5). (C) Representative Western blotting pictures for B. (D) ROS formation (n = 5). (E) The 3-nitrotyrosine formation (n = 5; *P < 0.05 vs. LG/EMP group). Data are expressed as mean ± SEM.