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. 2019 Nov 4;116(47):23743–23752. doi: 10.1073/pnas.1912625116

Fig. 3.

Fig. 3.

Hyperglycemia-induced persistent oxidative stress and SOD2 suppression were restored by expression of SOD2/Egr1 lentivirus. The human ACS-5003 neurons were treated by either 8-d LG [LG(8 d)] or 4-d HG plus 4-d LG [HG(4 d)+LG(4 d)] or the cells were infected on day 4 by SOD2 lentivirus [HG(4 d)+LG(4 d)/↑SOD2] or Egr1 lentivirus [HG(4 d)+LG(4 d)/↑Egr1]; the cells were then used for further analysis: (A) ROS formation (n = 4), (B) ChIP analysis on SOD2 promoter (n = 4), and (C) SOD2 mRNA levels [n = 4; *P < 0.05 vs. LG(8 d) at day 0 group; P < 0.05 vs. LG(8 d) at day 1 group]. Data are expressed as mean ± SEM.