Fig. 2.

MHCII⁺ keratinocytes participate in lymphocyte education. (A–G) CD49f⁺Sca-1⁺MHCII⁻ keratinocytes from unassociated control (Ctrl MHCII⁻), S. epidermidis-associated mice (S. epi MHCII⁻) and CD49f⁺Sca-1⁺MHCII⁺ keratinocytes from S. epidermidis-associated mice (S. epi MHCII⁺) were sorted from CD45⁻CD34⁻CD31⁻ epidermal cell suspension at day 14 postassociation and analyzed by RNA-seq. (A) PCA of RNA-seq performed on CD49f⁺Sca-1⁺MHCII⁻ keratinocytes from unassociated control or S. epidermidis-associated mice and CD49f⁺Sca-1⁺MHCII⁺ keratinocytes from S. epidermidis-associated mice. Ellipses represent 95% confidence intervals of the mean. (B–E) Heatmaps showing expression of genes encoding antimicrobial peptides in 4 replicates per condition (B), genes associated with lymphocyte recruitment (C), antiviral responses (D), and Ahr and IFN signaling (E). (F) IPA of genes up-regulated in CD49f⁺Sca-1⁺MHCII⁺ keratinocytes vs. CD49f⁺Sca-1⁺MHCII⁻ keratinocytes from S. epidermidis-associated mice (G) Heatmap showing expression of genes implicated in antigen presentation. (H) Expression of H2Kb by CD49f⁺Sca-1⁺MHCII⁺ keratinocytes and CD49f⁺Sca-1⁺MHCII⁻ keratinocytes in control mice or day 14 after association with S. epidermidis.