Fig. 5.

ER stress alters cell morphology, actin turnover dynamics, and intracellular actin filament organization in THP1 CD1d cells. Cells were treated with appropriate control solvent (DMSO or Glycerol) or ER stress-inducing drugs (thapsigargin 0.03 μM or 1 μg/mL SubAB5). (A and B) microscopic images of actin fluorescence for quantification of actin distribution area. (i) Image of a cell treated with the solvent control; (ii) the corresponding ER-stress condition. (C and D) FRAP analysis of fluorescence recover of control or ER-stressed THP1 cells to measure actin filament polymerization dynamics. Panel (i) shows half-time of fluorescence recovery and (ii) compares the mobile an immobile actin fractions upon treatment. The data represent n ≥ 3 biological replicates. *P < 0.05, **P < 0.005, ****P < 0.0001 by unpaired, 2-tailed t test.