Fig. 4.

RNA-seq of human epithelial colon cells with MRTFB depletion identified target genes of its transcriptional activity. (A) Western blot analysis results showed efficient knockdown of MRTFB expression in hCEC1 cells at different time points. (B) Venn diagram of comparison of significantly up-regulated genes among MRTFB siRNA samples. (C) Two known oncogenes were among the 6 common up-regulated genes that are potentially direct targets of MRTFB transcriptional activity. (D) PCR results validated the significant expression level changes of the 6 common up-regulated target genes of MRTFB identified by RNA-seq. All RT-qPCR analyses were performed 3 times (all P < 0.05).