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. 2019 Nov 6;116(47):23822–23828. doi: 10.1073/pnas.1909917116

Fig. 5.

Fig. 5.

Loss of NAMPT impairs adrenergic-mediated lipolytic activity through inactivation of caveolin-1 in WAT. (A) Plasma concentrations of glycerol and FFA and blood glucose concentrations before and after an injection of CL-316243 (1 mg/kg) (n = 4 to 8 per group). (B) WAT depots were incubated in the presence or absence of ISO for 2 h (n = 5 to 8 per group). (C) Western blot analysis of CAV1 and IRβ in WAT (n = 4 to 5 per group). (D) Western blot analysis of phospho-PLIN1 (Ser522), PLIN1, phospho-HSL (Ser563), and HSL in WAT after CL-316243 injection (n = 4 per group). NAD+ concentrations (E) and Cav1 gene expression (F) in OP9 adipocytes in the presence or absence of FK866, NMN, and Ex527 (n = 4 per group). (G) ISO-stimulated glycerol release from GFP (G) or Cav1 siRNA-treated OP9 adipocytes (n = 4 per group). #Repeated measures ANOVA revealed a significant group × time interaction (P < 0.05). Data were analyzed by Student’s unpaired t test (A–D) and one-way ANOVA with Tukey’s post hoc test (E–G). *P < 0.05. Values are means ± SEM.