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. 2019 Nov 4;116(47):23813–23821. doi: 10.1073/pnas.1912573116

Fig. 2.

Fig. 2.

GPR43 deficiency prevents body weight loss in the fasting condition. (A–C) Wild-type and Gpr43−/− mice were fasted and assessed for plasma acetoacetate (A), β-hydroxybutyrate (B), and SCFA (C) levels at 0, 24, and 48 h (n = 7 to 12). (D and E) Body weight changes were monitored for 48 h in the fasting condition. Relative body weight change (D), fat mass (E, Left) and representative macroscopic appearance (E, Right) were measured (n = 10 to 12). (Scale bar: 1 cm.) Open squares represent values for fasting mice for 48 h; closed squares, values for nonfasting mice. (F–I) Blood glucose (F), plasma TG (G), NEFA (H), and total cholesterol (I) levels were measured after 0, 24, and 48 h of fasting (n = 8 to 12). (J and K) Energy expenditure (J) and respiratory exchange ratio (RER; K) of fasted-mice in time course changes (n = 7). **P < 0.01; *P < 0.05, Student’s t test. ##P < 0.01; #P < 0.05 compared with wild-type mice, Student’s t test. All data are presented as mean ± SEM.