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. 2019 Nov 4;116(47):23636–23642. doi: 10.1073/pnas.1901732116

Fig. 1.

Fig. 1.

Identification of a forebrain enhancer. (A) Genomic locations of the 6 known forebrain enhancers in the Shh regulatory region. SBE1 and SBE5 are intronic enhancers in the Shh and Lmbr1 genes, respectively. The other 4 enhancers are located in the intergenic region. (B) ChIP-seq data on the embryonic brain at E14.5 with anti-H3K27ac and anti-H3K4me1 antibodies uploaded in the UCSC genome browser. Several signal peaks were found upstream of the Shh locus. (Lower) 6 plots showing an enlarged view of the cluster harboring 3 forebrain enhancers, shaded in 2 first (Upper) plots. (Bottom) 4 plots showing phylogenetically conserved regions detected by Phylop and PhastCons. (C–G) Transgenic reporter expression driven by e1, e2, e3, e4, and SBE2 genome fragments at E10.5. The number of LacZ-positive embryos among the total number of embryos carrying a transgene is indicated (Bottom Right). (H) A sagittal section of the e2 transgenic brain. Di, diencephalon; Mb, midbrain; Nc, notochord; Lu, lung; Us, upper stomach; Rp, Rathke’s pouch; I, infundibulum. (Scale bar, 0.5 mm.)