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Characterization of iron acquisition proteins. (A) Growth curves of WT, ∆FBP1, and ∆FBP1 complement cultures grown in Aquil media under 12-h illumination, 10 nM total Fe, 100 µM EDTA, and 100 nM DFOB. Error bars represent ±SD of biological triplicate cultures. (B) Cells expressing mCherry tagged FBP1 and YFP-tagged FRE2 were imaged at 1,000× magnification after acclimation to low-iron conditions.
