Fig. 1.

PCA of expression profiles from primary EC of wild-type heart and kidney capillaries obtained immediately after isolation (day 0) identified 4,783 differentially expressed genes (FDR < 10%) between tissue origins. In vitro expansion for 3 d resulted in significant shifts in expression programs, with the number of differentially expressed genes being reduced to 2,397 between heart and kidney ECs, indicating phenotypic drift occurring in the absence of a native microenvironment; n = 3 biologic replicates per condition.