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. 2019 Nov 5;116(47):23790–23796. doi: 10.1073/pnas.1908529116

Fig. 2.

Fig. 2.

Delayed immune responses in microglia from heterozygous Abca7 knockout mice after LPS stimulation. Primary cultures of microglia were prepared from control (white) and Abca7+/− (gray) mice. The ΔΔCt values of Il6 (A), Tnf (B), and Il1b (C) relative to Hprt were measured by qRT-PCR in microglia at 3, 6, and 12 h after culture with LPS (10 ng/mL). Phosphorylation of NF-κB (D), p38 MAPK (E), and ERK (F) was analyzed by Western blotting at 15, 30, and 60 min after culture with LPS. Relative ratios to control microglia without LPS are shown as mean ± SEM (n = 3/each). *P < 0.05; ***P < 0.001, Student t test at each time point.