Fig. 4.

Influence of ABCA7 haplodeficiency on CD14 in App^{NL-G-F/NL-G-F} mice. (A) Brain sections from App^{NL-G-F/NL-G-F};control (n = 14) and App^{NL-G-F/NL-G-F};Abca7^+/− mice (n = 12) were immunostained for Iba-1, CD14, and EEA1 at age 3 mo. Representative images from the dentate gyrus region are shown. (Scale bars: 10 μm.) (B–D) Localization of CD14-positive (B) and EEA1-positive (C) compartments in Iba-1–positive cells was quantified in the cortex (CX), CA1, and dentate gyrus (DG) of the mice using ImageJ. (D) The ∆∆Ct values of Cd14 relative to Hprt were measured by qRT-PCR in the cortex and hippocampus (HP). Relative ratios to App^{NL-G-F/NL-G-F};control mice are shown as mean ± SEM, *P < 0.05, Student t test.