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. 2019 Nov 13;10:1349. doi: 10.3389/fphar.2019.01349

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Copyright © 2019 Yin, Liu, Li, Wang, Tang and Huang

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Adenoviral Id2 delivery ameliorated cardiac remodeling post-MI. (A, B) Representative Masson’s trichrome staining (A) and histogram of connective tissue percentage (B) of hearts. Blue staining indicates connective tissue (n = 6). Scale bars represent 100 um. (C) Representative HE staining images (n = 6). Scale bars indicate 100 um. (D) immunofluorescence images of myofibroblasts derived from CFs (n = 6). Green, vimentin; red, α-SMA; blue, nuclei. Scale bars represent 100 um. (E, F) Western blot analysis of protein expressions in rat hearts among the four groups (n = 3). β-actin was used as the loading control. Data represent means ± SEM. *,P < 0.05, vs sham group; # ,P < 0.05, vs MI group and GFP group; in panel (E) including α-SMA, Collagen I, Collagen III in both MI and GFP group; in panel (E) including MMP2, TIMP-1 in both MI and GFP group.

Inline graphic — Adenoviral Id2 delivery ameliorated cardiac remodeling post-MI. (A, B) Representative Masson’s trichrome staining (A) and histogram of connective tissue percentage (B) of hearts. Blue staining indicates connective tissue (n = 6). Scale bars represent 100 um. (C) Representative HE staining images (n = 6). Scale bars indicate 100 um. (D) immunofluorescence images of myofibroblasts derived from CFs (n = 6). Green, vimentin; red, α-SMA; blue, nuclei. Scale bars represent 100 um. (E, F) Western blot analysis of protein expressions in rat hearts among the four groups (n = 3). β-actin was used as the loading control. Data represent means ± SEM. *,P < 0.05, vs sham group; # ,P < 0.05, vs MI group and GFP group; in panel (E) including α-SMA, Collagen I, Collagen III in both MI and GFP group; in panel (E) including MMP2, TIMP-1 in both MI and GFP group.