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. 2019 Nov 13;10:1349. doi: 10.3389/fphar.2019.01349

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Copyright © 2019 Yin, Liu, Li, Wang, Tang and Huang

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Id2 reduced carmyocyte apoptosis post-MI. (A, B) Representative TUNEL staining. The apoptotic cells show yellow-brown nuclei, while the normal cells exhibit light blue nuclei. (n = 6). Scale bars represent 100 um. (C) The protein levels of c-caspase3, c-caspase9, bax and bcl-2 in rat hearts (n = 3). (D) The apoptotic cell rate in four groups. (E) Immunofluorescence images of cardiomyocytes among four groups in vivo (n = 6). Red, c-caspase3; blue, nuclei. Scale bars represent 100 um. β-actin was used as the loading control. Data represent means ± SEM. *P <0.05, vs control group. ^#P <0.05, vs MI group and GFP group; in panel (C) including c-caspase3, c-caspase9, bax and bcl-2 in both MI group and GFP group; in panel (C) including c-caspase3, c-caspase9, and bcl-2 in Id2 group.

Inline graphic — Id2 reduced carmyocyte apoptosis post-MI. (A, B) Representative TUNEL staining. The apoptotic cells show yellow-brown nuclei, while the normal cells exhibit light blue nuclei. (n = 6). Scale bars represent 100 um. (C) The protein levels of c-caspase3, c-caspase9, bax and bcl-2 in rat hearts (n = 3). (D) The apoptotic cell rate in four groups. (E) Immunofluorescence images of cardiomyocytes among four groups in vivo (n = 6). Red, c-caspase3; blue, nuclei. Scale bars represent 100 um. β-actin was used as the loading control. Data represent means ± SEM. *P <0.05, vs control group. ^#P <0.05, vs MI group and GFP group; in panel (C) including c-caspase3, c-caspase9, bax and bcl-2 in both MI group and GFP group; in panel (C) including c-caspase3, c-caspase9, and bcl-2 in Id2 group.