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. 2019 Nov 13;10:1349. doi: 10.3389/fphar.2019.01349

Figure 7.

Figure 7

NRVMs treated with hypoxia. (A–E) Flow cytometry of NRVMs’ apoptosis rate after treated with different hypoxia time (n = 3). (A) 0 h, (B) 4 h, (C) 6 h, (D) 8 h, (E) 12 h. (F) The mRNA levels of rat Id2 in NRVMs along with different hypoxia time. (G) The mRNA levels of bax/bcl, c-caspase3 and c-caspase9 in NRVMs with different hypoxia time. (H. I) qRT-PCR and Western blot were performed to detect the mRNA levels (H) or the protein expressions (I) of bax/bcl, c-caspase3 and c-caspase9 in NRVMs treated with hypoxia for 6 h. (J) The protein level of HIF-1α in NRVMs in four groups. β-actin was used as the loading control. Data represent means ± SEM (n = 3). *P <0.05, vs control groups or 0 h; #P <0.05, vs H groups and H+G groups; Inline graphic in panel (G) including c-caspase3, c-caspase9, bax/bcl-2 in both 6 h and 8 h; Inline graphic in panel (H) including c-caspase3, c-caspase9, bax/bcl-2 in both H group and H+G group; Inline graphic in panel (H) including c-caspase3, c-caspase9, and bax/bcl-2 in Id2 group; Inline graphic in panel (I) including c-caspase3, c-caspase9, bax and bcl-2 in both H group and H+G group; Inline graphic in panel (I) including c-caspase3, c-caspase9, bax, and bcl-2 in Id2 group.