Figure 1.

Bioactivation of PL and PL-probes by hPLK

(A) To generate biologically active vitamin B₆, PL is phosphorylated to PLP by hPLK inside the cell. PLP is then bound as an internal aldimine to PLP-DEs.

(B) Chemical structures of PL and PL1–PL3.

(C) UV-vis spectra of PLP and PLP-probes. Curves were recorded for equal vitamer and probe concentrations.

(D) Kinetic parameters (n = 3 biological replicates; data presented as means ± SEM) for PL and PL-probe turnover.

See also Figure S1.