Figure 4.

In Situ Target Screening of Penicillamine

(A and B) PL1-labeled HEK293 cells were incubated with 10 mM of l-Pen (A) and d-Pen (B) for 2 h, respectively. Volcano plots representing t test results of the competition compared with the PL1-labeled control revealed significant depletion of PLP-DEs (blue) (n = 3 biological replicates). Cut-off values were defined as the depletion factor of log₂ = −1 (2-fold depletion) and −log₁₀ (p value) of 1.3 (solid lines).

(C) Mechanism of thiazolidine adduct formation of Pen with PLP (P = OPO₃²⁻).

(D) UV-vis spectra of PLPBP dimer incubated with increasing concentrations of l-Pen.

See also Figure S2 and Table S2.