Fig. 10.

Retinal development in Aey69 mutants. a) The ganglion cell specific marker BRN3 was used to characterize the early retina developmental changes and associated hyperproliferative events. The arrows in the mutant retina at E11.5 clearly indicate an early overexpression of BRN3-positive retinal cells. This overexpression does not affect the expansion of the BRN3 positive cells to the prospective ganglion cell layer in mutant retina at E15.5 similar to the wild type (marked by arrows in the respective sections). b) OTX2 was used to characterize the early changes in Aey69 mutant retina at the stages of E11-5-E13.5. The results indicate an early appearance of OTX2-positive retinal cells in the mutant at E11.5 and E12.5 (indicated by white arrows at the respective stage). n = 3, for each embryonic stage; bars indicate 100 μm; L, lens; R, retina; ON, optic nerve.